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Table 2 Effect of colony stimulating factor 2 (CSF2) and sex on survival of blastocysts following vitrification as evaluated by re-expansion of the blastocoele and initiation of hatching from the zona pellucida after 24 h of culture post-thawing

From: Determinants of survival of the bovine blastocyst to cryopreservation stress: treatment with colony stimulating factor 2 during the morula-to-blastocyst transition and embryo sex

Treatment Sex Number of blastocysts Re-expansion (%)a Number of blastocystsb Hatching (%)a
Vehicle Female 94 84.0 ± 3.8 83 52.5 ± 6.9
CSF2 Female 115 87.8 ± 3.0 102 51.1 ± 5.8
Vehicle Male 142 82.4 ± 3.2 125 58.8 ± 6.5
CSF2 Male 149 84.6 ± 3.0 137 58.7 ± 5.8
P values
CSF2    0.525   0.882
Sex    0.582   0.160
CSF2 x sex    0.810   0.902
  1. aData are least-squares means ± SEM
  2. bNumber is lower than for re-expansion because embryos that were hatching or hatched before vitrification were excluded