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Fig. 1 | CABI Agriculture and Bioscience

Fig. 1

From: Optimized rice transformation protocol for transformation of the blast susceptible Indica rice accession CO39

Fig. 1

Regeneration of CO39 calli is inhibited by presence of antibiotics. A Calli after 14 days on regeneration media MSRMa-II containing antibiotics. Calli browning and poor shoot regeneration can be observed. B Callus after 9 days on MSRMa-II media excluding antibiotics. C Callus after 14 days on MSRMa-II media excluding antibiotics. D Close up image of regenerating callus 14 days after transfer to MSRMa-II excluding antibiotics. E Image of regenerated shoots of different ages after transfer to rooting media, MROM containing 30 mg/l hygromycin. Shoots were transferred to rooting media after forming 1–2 cm shoots on regeneration media excluding antibiotics. A shoot from a putative transformation event at the back of the rooting media is a non-transgenic escape and is killed by transfer to hygromycin containing rooting media. For scale, petri dishes are 90 mm wide and jam jar in E is 50 mm wide

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CABI Agriculture and Bioscience

ISSN: 2662-4044