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Fig. 2 | CABI Agriculture and Bioscience

Fig. 2

From: Optimized rice transformation protocol for transformation of the blast susceptible Indica rice accession CO39

Fig. 2

Example of hygromycin leaf-tip assay for segregating T1 population. 17/63 and 15/63 T1 plants from the segregating T1 of LOC_Os12g22880_1 and LOC_Os12g22880_8 transformation events, respectively were likely homozygous non-transgenic individuals based on necrosis of leaf tissue* indicative of hygromycin susceptibility. The results of the chi-square test show that the observed segregation ratio does not differ significantly (p > 0.05) from the expected segregation ratio (1/4) of a single-copy, or single locus transformation event. Color saturation and color temperature have been adjusted to improve visibility of leaves. Original image is presented in Additional file 1: Fig. S2

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CABI Agriculture and Bioscience

ISSN: 2662-4044