Fig. 4

Scaled production of pigs containing modified allele of CD163. A. High genetic merit gilts from nucleus are synchronized and artificially inseminated with semen from nucleus boars. Post fertilization, one-cell zygotes collected from donor gilts are microinjected with the dual-guide RNP complex (RNP C + D) identified in the process from Fig. 3 and then 25–35 two-cell stage zygotes transplanted into synchronized surrogate gilts. Approximately 115 days later these surrogates farrow litters ranging from 6 to 12 piglets. B. Cartoon depicting genetic makeup of pigs containing wild-type (tan), mosaic, heterozygous or homozygous modified CD163 allele (green)