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Fig. 2 | CABI Agriculture and Bioscience

Fig. 2

From: Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells

Fig. 2

Effect of LPS on mRNA expression of factors that mediate estradiol synthesis in granulosa cells from small/medium follicles. Bovine granulosa cells from small/medium diameter (2–8 mm) follicles were exposed to control medium (light bars) or LPS (1–104 ng/mL; dark bars) for 24 h. Gene expression of AMH (A), ESR1 (B), FSHR (C), LHCGR (D), STAR (E), HDAC1 (F), and HDAC10 (G) were quantified by PCR (n = 10). Data were log transformed for normality and a general linear model with fixed effect of LPS dose and random effect of replicate followed by least significant difference pairwise comparisons were used to analyze each dose compared to the medium alone control. Data are presented as the estimated marginal means ± S. E. M. There was no statistical differences detected for any gene

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