Fig. 3
From: Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells
Acute effects LPS on mRNA expression of inflammatory mediators and estradiol synthesis in granulosa cells from small/medium follicles. Bovine granulosa cells from small/medium diameter (2–8 mm) follicles were exposed to control medium (light bars) or 104 ng/mL LPS (dark bars) for 0.5, 2, 4, 8, or 12 h. Gene expression of CXCL8 (A), IL6 (B), HSD17B1 (C), CYP19A1 (D) and STAR (E) were quantified by qPCR (n = 6). Accumulation of 17β-estradiol (F) was quantified at 12 h using ELISA (n = 6). Gene expression data were log transformed and data were analyzed using a general linear model with replicate as a random effect and the fixed effects of LPS, time, and the interaction of LPS and time followed by least significant difference pairwise comparisons. Data are presented as the estimated marginal means ± S. E. M. Statistical significance for each test is presented when significant. * indicates P ≤ 0.05 compared to medium alone at a specific timepoint