Fig. 6
From: Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells
Effect of LPS on granulosa cell CEBPB mRNA expression and CEBPβ abundance. Bovine granulosa cells from small/medium (A, B) follicles or large (C-D) follicles were exposed to control medium (light bars) or 104 ng/mL LPS (dark bars) for 24 h. Gene expression of CEBPB (A, C) was quantified by qPCR (n = 6–9), and protein abundance of CEBPβ (B–D) was evaluated by western blot (n = 4). Gene expression data were log transformed for normality and a general linear model with fixed effect of LPS and random effect of replicate. Data are presented as the estimated marginal means ± S. E. M. * indicates P ≤ 0.05 compared to medium alone control. Western blot of total protein is presented in Additional file 1: Fig. S1C, D and uncropped blots are presented in Additional file 1: Fig. S2C, D