Table 1 Primer sequences used for real time RT-PCR
From: Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells
Gene | Primer sequence | Accession number |
|---|---|---|
AMH | 5′-GTGGTGCTGCTGCTAAAGATG 3′-TCGGACAGGCTGATGAGGAG | NM_173890.1 |
ACTB | 5′-CAGAAGCACTCGTACGTGGG 3′-TTGGCCTTAGGGTTCAGGG | NM_173979.3 |
CEBPB | 5′-ACAGCGACGAGTACAAGATCC 3′-GACAGTTGCTCCACCTTCTTCT | NM_176788.1 |
CXCL8 | 5′-GCAGGTATTTGTGAAGAGAGCTG 3′-CACAGAACATGAGGCACTGAA | NM_173925.2 |
CYP17A1 | 5′-CTCCAGCATTGGCGACCTTA 3′-GAAGCGCTCGGGCATGAA | XM_024985958.1 |
CYP19A1 | 5′-CGCAAAGCCTTAGAGGATGA 3′-ACCATGGCGATGTACTTTCC | NM_174305.1 |
FSHR | 5′-GCAGTCGAACTGAGGTTTGTT 3′-TTGGAGAACACGTTTGCCTCT | NM_174061.1 |
GAPDH | 5′-AGGTCGGAGTGAACGGATTC 3′-ATGGCGACGATGTCCACTTT | NM_001034034.2 |
HDAC1 | 5′-TTACGACGGGGATGTTGGAA 3′-GGCTTTGTGAGGGCGATAGA | NM_001075460.1 |
HDAC10 | 5′-CTCGGCTTCACTGTCAACCT 3′-TCAGGGTCGAACTCAAAGGC | NM_001037444.2 |
HSD17B1 | 5′-CGTGAGGGATGCAGATTCCA 3′-GTTACACACCAGCACGTCCA | NM_001102365.1 |
IL6 | 5′-ATGACTTCTGCTTTCCCTACCC 3′-GCTGCTTTCACACTCATCATTC | NM_173923.2 |
LHCGR | 5′-TGCCTTTGACAACCTCCTCAAT 3′-GATGCTTAGGTATTTTAACCGAGG | NM_174381.1 |
PTPRC | 5′-CTCGATGTTAAGCGAGAGGAAT 3′-TCTTCATCTTCCACGCAGTCTA | NM_001206523.1 |
STAR | 5′-AGAAGGGTGTCATCAGAGCG 3′-TGGTCCTTGAGGGACTTCCA | NM_174189.3 |