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Fig. 1 | CABI Agriculture and Bioscience

Fig. 1

From: Diversity in mitotic DNA repair efficiencies between commercial inbred maize lines and native Central American purple landraces

Fig. 1

Recovery from DNA damage in US maize inbred lines (B73 and Mo17) and purple Central American landraces (Orotina Congo [C], Jocopilas [J], P1-Pujagua Santa Cruz, P2-Pujagua La Cruz, Talamanca [T]) as observed by comet assay. The comet assay is a gel electrophoresis-based method that can be used to measure DNA damage in individual cells. If negatively charged DNA contains breaks, those broken ends migrate toward the anode and form a tail; thus, the amount of DNA in the tail is related to the severity of damage. In this study, seedlings were treated with zeocin (100 μg−mL) for 24 h. DNA damage was compared to an untreated control. 1 h refers to samples collected after being washed and allowed to recover for 1 h in water (n = 3, 3 biological samples, 3 technical replicates and 50 nuclei per replicate)

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CABI Agriculture and Bioscience

ISSN: 2662-4044