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Table 2 Effect of solvent extraction method on the total phenolic content, total flavonoid content, total tannin content, percent of 2,2-diphenyl-1-picrylhydrazyl inhibition and ferric reducing antioxidant power activity absorbance of Rosmarinus officinalis leaves

From: Evaluation of seasonal variation, effect of extraction solvent on phytochemicals and antioxidant activity on Rosmarinus officinalis grown in different agro-ecological zones of Kiambu County, Kenya

Extracting solvent

TPC (mg/g GAE)

TFC (mg/g RUTE)

TTC (mg/g TAE)

DPPH inhibition (%)

FRAP activity (absorbance)

80%

ethanol

39.71 ± 6.77 ns

34.72 ± 6.51 c

15.02 ± 1. 25 a

80.00 ± 12.14 a

0.58 ± 0.4 a

80%

methanol

24.91 ± 5.15 ns

16.86 ± 2.80 a

4.27 ± 1.48 b

82.35 ± 12.1 a

0.74 ± 0.11 ab

Aqueous

24.91 ± 7.30 ns

117.22 ± 12.64 b

19.88 ± 4.59 a

33.03 ± 4.69 b

0.84 ± 0.05 b

p-value

0.219

0.0001

0.0002

0.0001

0.339

  1. Results are means from leaves harvested from four agro-ecological zones in Kenya. Values bearing the same letter in the column are not statistically different (P < 0.05). Values are expressed as mean ± Standard Deviation
  2. TPC total phenolic content, TFC total flavonoids content, TTC total tannins content, DPPH diphenyl-1-picrylhydrazyl inhibition, FRAP ferric reducing antioxidant power, GAE gallic acid equivalent, RUTE rutin equivalent, TAE tannic acid equivalent, ns no significant differences
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CABI Agriculture and Bioscience

ISSN: 2662-4044