Fig. 4
From: Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells
Excess androstenedione does not prevent LPS-mediated estradiol accumulation of granulosa cells from small/medium follicles. Bovine granulosa cells from small/medium diameter (2–8 mm) follicles were exposed to control medium (light bars) or 103 ng/mL LPS (dark bars) for 24 h in medium supplemented with 0, 1 or 100 μM androstenedione (A4). Accumulation of 17β-estradiol (A) was quantified using ELISA and gene expression of HSD17B (B), CYP19A1 (C) and STAR (D) were quantified by qPCR (n = 6). Data were analyzed using a general linear model with replicate as a random effect and the main effects of LPS, androstenedione, and the interaction of LPS and androstenedione followed by least significant difference pairwise comparisons. Data are presented as the estimated marginal means ± S. E. M. Statistical significance for each test is presented when significant and * indicates P ≤ 0.05