Fig. 7
From: Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells
Effect of LPS on CEBPβ nuclear translocation in granulosa cells from small/medium follicles. Bovine granulosa cells from small/medium diameter (2–8 mm) follicles were exposed to control medium (light bars) or 104 ng/mL LPS (dark bars) for 6 or 24 h. Nuclear localization of CEBPβ was assessed using immunocytochemistry immediately after the treatment period (n = 5 for each time point). (A) Representative images show individual nuclear and CEBPβ staining and a merge image showing nuclear (blue) and CEBPβ staining (green). Each scale bar represents 50 μm. (B) Nuclear translocation of CEBPβ was quantified using ImageJ. Data were analyzed using a general linear model with replicate as a random effect and the main effects of LPS and time, and presented as the estimated marginal means ± S. E. M