Fig. 8
From: Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells
Effect of LPS on CEBPβ nuclear translocation in granulosa cells from large follicles. Bovine granulosa cells from large diameter (> 8 mm) follicles were exposed to control medium (grey bars) or 104 ng/mL ultrapure LPS (black bars) for 6 h or 24 h. Nuclear localization of CEBPβ was assessed using immunocytochemistry immediately after the treatment period (n = 7 foreach time point). (A) Representative images show individual nuclear and CEBPβ staining and a merge image showing nuclear (blue) and CEBPβ staining (green). Each scale bar represents 50 μm. (B) Nuclear translocation of CEBPβ was quantified using ImageJ. Data were analyzed using a general linear model with replicate as a random effect and the main effects of LPS and time, and presented as the estimated marginal means ± S. E. M