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Table 1 Primers used for cDNA cloning, quantitative real-time PCR (RT-qPCR), and double-stranded RNA (dsRNA) synthesis

From: Ecdysone receptor strongly influences larval–pupal–adult transition and melanization in Tuta absoluta

Primer name

Primer sequence (5′→3′)

Amplicon length/bp

EcR- F

TCAACAAAGTTTAAGTTTTC

1860

EcR- R

ACGATCTGAATCTCCTTG

dsEcR-F

taatacgactcactatagggGCGACCAACCAAGCCTACAC

406

dsEcR-R

taatacgactcactatagggAAGACACATCGGCGACATCC

qEcR-F

AAGTGACTTCGTCTAGTGCGTTGG

119

qEcR-R

GCTGTTGATGCCGTCGTCGTAG

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CABI Agriculture and Bioscience

ISSN: 2662-4044