Fig. 6

Regulations of WRKY in various AHL-primed plants after challenge with flg22. Two-week-old A. thaliana was treated with either 6 µM oxo-C6-HSL, oxo-C8-HSL, oxo-C12-HSL, oxo-C14-HSL, or their mixture (AHL mix) for 72 h. Total RNA was extracted prior the challenge (72 h) as well as 2 h (74 h) and 24 h (96 h) after an additional challenge with 100 nM flg22. Expression of ubiquitin ligase (At5g25760) was used for normalization. Boxes represent the interquartile range between the first and the third quartile and the middle line marks the median, whiskers indicate 1.5 × interquartile range. Each treatment and time point had four biological replicates. Comparisons are indicated by the horizontal lines above bars, statistical analysis was performed with Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001