Fig. 4
Time-kill graphs to show synergistic potential in which flasks containing SDB broth were added with α-Phellandrene, antifungal drugs and their combinations and then inoculated with the respective fungal cells. a Plots of mean values for log10 of the numbers of CFU/ml versus time tested against C. albicans ATCC90028 at the concentrations: 1MIC α-Phellandrene, 1 MIC Fluconazole and 1MIC α-Phellandrene + 1 MIC fluconazole. α-phellandrene was combined with fluconazole, the results were synergistic (a 2-log10 reduction in colony count after 16 h with the combination, compared to the most active medicine alone. b The same limiting fungal growth pattern observed in plots of mean values for log10 of the numbers of CFU/ml versus time tested against C. albicans ATCC90028 at the concentrations: 1MIC α-Phellandrene, 1 MIC Amphotericin B and 1MIC α-Phellandrene + 1 MIC Amphotericin B. c Plots of mean values for log10 of the numbers of CFU/ml versus time tested against C. albicans MTCC277 at the concentrations: 1MIC α-Phellandrene, 1 MIC Fluconazole and 1MIC α-Phellandrene + 1 MIC fluconazole. α-Phellandrene and fluconazole, alone were shown to have no effect. α-phellandrene was combined with fluconazole, the results were synergistic. d Plots of mean values for log10 of the numbers of CFU/ml versus time tested against C. albicans MTCC277 at the concentrations: 1MIC α-Phellandrene, 1 MIC Amphotericin B and 1MIC α-Phellandrene + 1 MIC Amphotericin B shows similar limiting fungal growth pattern in respect of combination