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Fig. 9 | CABI Agriculture and Bioscience

Fig. 9

From: Synergistic potential of α-Phellandrene combined with conventional antifungal agents and its mechanism against antibiotic resistant Candida albicans

Fig. 9

Micrographs taken with a FESEM of untreated and treated Candida cells after 48 h: a untreated C. albicans (ATCC90028) cells with typical round smooth surfaces (× 12,000), b untreated C. albicans (MTCC277) cells with usual oval-shaped and smooth outer layers (× 4,000) (c) clustered and damaged ATCC90028 Candida strain cells that had been treated with α-Phellandrene (× 12,000), (d) inflated and distorted C. albicans cells ATCC90028 that had been treated with fluconazole (× 6000), (e) creation of pits and deformity in amphotericin B treated ATCC90028 candida strain cells (× 12,000), (f) rupture of the fungal membrane and a total breakdown of the integrity of the membrane in α-Phellandrene + fluconazole treated ATCC90028 candida strain cells (× 12,000), (g) intrinsic material of the fungal cells released and the aggregate cells showed as sludge when treated with α-Phellandrene and amphotericin B against ATCC90028 Candida strain cells (× 6,000), (h) contraction and deformation in α-Phellandrene treated C. albicans MTCC277 cells (× 6000), (i) fluconazole-treated MTCC277 Candida strain cells displaying substantial folds and deformities (× 6000), (j) pits like framework formed in amphotericin B treated MTCC277 Candida strain cells (× 6000), (k) lysed cells and only the traces of lysed fungus in α-Phellandrene + amphotericin B treated MTCC277 Candida strain cells (× 6000), (l) extensive cell damage in fluconazole treated MTCC277 Candida strain cells (× 12,000). Arrows in red show any specific observations that have been found. The resolutions/magnifications for the topographical information and the elemental information are represented as × 4000, × 6000 × 12,000 (the reader is directed to the web version of this article for an interpretation of the symbols to color that are contained within this figure legend).

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