Table 2 List of SSR primers used for the study
No | Marker ID | Repeat Motif | FPr (5′-3′) | Tm(℃) | Size(bp) | RPr (5′-3′) | Tm(℃) | Size(bp) | PSize(bp) |
|---|---|---|---|---|---|---|---|---|---|
1 | PhE842 | (CT)9 | GAGGCTCCTTGGACATCACC | 60.107 | 20 | CCGACGAATAAGGCAGGCTT | 60.46 | 20 | 202 |
2 | PhE195 | (TCC)5 | CGAAGTGGAAGAGGTCGTAG | 57.454 | 20 | CTTTCCCTTGCCTCCCTTC | 57.73 | 19 | 237 |
3 | PhE453 | (AGAGG)6 | CATTCTGTTGATGGCGCTAT | 56.278 | 20 | CAGGGAGAACGACCAACTAC | 57.64 | 20 | 201 |
4 | PhE82 | (GA)21 | GGAGCGCAAATCCCATAAAG | 57.218 | 20 | CTTGCTTCGTTGCCAGTATC | 57.19 | 20 | 243 |
5 | PhE973 | (GA)14 | TTTGGGAGAGGGATTTTGCT | 57.001 | 20 | AACTCAGTGCATCAGATCGT | 56.95 | 20 | 205 |
6 | PhE317 | (TC)10 | ACTGATATTCACCCTGCAGT | 56.219 | 20 | ACAGTGGCGAAGATGAAGAT | 56.93 | 20 | 212 |
7 | PhE196 | (AG)9 | GGCTGCATAGTTCAAAGGAC | 56.786 | 20 | TGAGCGAACTACCCAAACTT | 57.08 | 20 | 218 |
8 | PhE734 | (TC)6 | TTACAATTTGAGGGCCCTGT | 57.009 | 20 | CATCTGGGTGCTGTTCTAGT | 56.93 | 20 | 206 |
9 | PhE360 | (CT)8 | CGAGGAGCCACTGATCTCAC | 59.898 | 20 | TCTCTTCCATCCCGAACCCT | 59.96 | 20 | 200 |
10 | Oxa1 | (CCT)5 | GCCTCCTTGAGATCCTCCTT | 59.778 | 20 | TGCAGCAGCAAGAACAGC | 60.01 | 18 | 202 |
11 | Oxa2 | (GCG)6 | TTTGGCGTAATCCCTGTAGC | 60.096 | 20 | TACCACAGCAGCAGCAACAC | 61.09 | 20 | 213 |
12 | Oxa3 | (GCC)6 | GACAGAAGCGGAAGTTGGAC | 59.851 | 20 | GAGAAGCAGCAGTGGAGAGC | 60.44 | 20 | 190 |
13 | Oxa4 | (TCG)6 | GAGGGACTGGATGATGGTGT | 59.774 | 20 | GAACGAGCCGTTCCAAATAG | 59.71 | 20 | 202 |
14 | Oxa5 | (GT)6 | CCCTGATGGTAGAAACACCG | 60.366 | 20 | AAAAATCCAAATGAGCATCAA | 57.24 | 21 | 214 |
15 | Oxa6 | (GGC)6 | TCGTTCTACATCCCGAGGTC | 60.073 | 20 | ACTGACCTGAACCGAACACC | 60.01 | 20 | 206 |
16 | Oxa7 | (CTC)6 | CTGTGACTGTGGATTGGTGG | 59.997 | 20 | CCTCTGACGCTGGAGCTG | 60.88 | 18 | 200 |